Two hundred newly hatched Cobb500 broiler chickens were randomly divided into five groups (n = 40). Their potential effects on broiler health, growth performance, and the immune system were evaluated in vivo. In addition to the previously reported poultry probiotic Bacillus subtilis KATMIRA1933, three strains with antimutagenic and antioxidant properties Bacillus subtilis KB16, Bacillus subtilis KB41, and Bacillus amyloliquefaciens KB54, were investigated. In this study, we focused on three probiotic strains isolated from the gastrointestinal tract of chickens bred in different housing types. One promising strategy for discovering Bacillus probiotics is selecting strains from the microbiota of healthy chickens and subsequent screening for potential biological activity. Probiotics are known for their beneficial effects on poultry health and wellbeing.
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